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|Title:||Analysis of T-DNA-mediated translational β-glucuronidase gene fusions|
Jean Pierre Hernalsteens
Henri De Greve
Vrije Universiteit Brussel
Universidad Complutense de Madrid
Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic
Scientific Institute of Public Health, Brussels
|Keywords:||Biochemistry, Genetics and Molecular Biology|
|Citation:||Plant Molecular Biology. Vol.36, No.2 (1998), 205-217|
|Abstract:||Three random translational β-glucuronidase (gus) gene fusions were previously obtained in Arabidopsis thaliana, using Agrobacterium-mediated transfer of a gus coding sequence without promoter and ATG initiation site. These were analysed by IPCR amplification of the sequence of gus and nucleotide sequence analysis. In one instance, the gus sequence was fused, in inverse orientation, to the nos promoter sequence of a truncated tandem T-DNA copy and translated from a spurious ATG in this sequence. In the second transgenic line, the gus gene was fused to A. thaliana DNA, 27 bp downstream an ATG. In this line, a large deletion occurred at the target site of the T-DNA. In the third line, gus is fused in frame to a plant DNA sequence after the eighth codon of an open reading frame encoding a protein of 619 amino acids. This protein has significant homology with animal and plant (receptor) serine/threonine protein kinases. The twelve subdomains essential for kinase activity are conserved. The presence of a potential signal peptide and a membrane-spanning domain suggest that it may be a receptor kinase. These data confirm that plant genes can be tagged as functional translational gene fusions.|
|Appears in Collections:||Scopus 1991-2000|
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