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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/18489
Title: Differentiation between Mycobacterium tuberculosis and Mycobacterium avium by amplification of the 16S-23S ribosomal DNA spacer
Authors: Arunnee Sansila
Poonpilas Hongmanee
Charoen Chuchottaworn
Somsak Rienthong
Dhanida Rienthong
Prasit Palittapongarnpim
Khon Kaen University
Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Mahidol University
Thailand Ministry of Public Health
Keywords: Medicine
Issue Date: 1-Sep-1998
Citation: Journal of Clinical Microbiology. Vol.36, No.9 (1998), 2399-2403
Abstract: Differentiation between Mycobacterium tuberculosis and M. avium is helpful for the treatment of disseminated mycobacterial infection in AIDS patients. This can traditionally be done by time-consuming biochemical tests or with Accuprobe. Previously, PCR restriction enzyme analysis (PCR-REA) of the 16S-23S rRNA gene spacer was shown to be able to identify a limited number of strains of Mycobacterium. In this study the method was improved by using more specific primers and was tested with 50 clinical isolates of M. tuberculosis and 65 clinical isolates of M. avium complex. Probes specific to the spacers of M. tuberculosis and M. avium were also tested. Both M. tuberculosis and M. avium could be reliably identified either by PCR-REA or by PCR-hybridization, with the results completely agreeing with those obtained by biochemical tests and with the Accuprobe, respectively. The method may therefore be useful as an alternative in-house method for identification of the bacteria.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0031855110&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/18489
ISSN: 00951137
Appears in Collections:Scopus 1991-2000

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