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Title: Functional characterizations of residues Arg-158 and Tyr-170 of the mosquito-larvicidal Bacillus thuringiensis Cry4Ba
Authors: Somphob Leetachewa
Saengduen Moonsom
Urai Chaisri
Narumol Khomkhum
Nonglak Yoonim
Wang Ping
Chanan Angsuthanasombat
Mahidol University. Institute of Molecular Biosciences
Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology
Keywords: Aedes aegypti;Bacillus thuringiensis;Cry4Ba toxin;Larvicidal activity;Peritrophic membrane;Open Access article
Issue Date: 2014
Citation: BMB Rep. Vol.47, No.10 (2014), 546-551.
Abstract: The insecticidal activity of Bacillus thuringiensis (Bt) Cry toxins involves toxin stabilization, oligomerization, passage across the peritrophic membrane (PM), binding to midgut receptors and pore-formation. The residues Arg-158 and Tyr-170 have been shown to be crucial for the toxicity of Bt Cry4Ba. We characterized the biological function of these residues. In mosquito larvae, the mutants R158A/E/Q (R158) could hardly penetrate the PM due to a significantly reduced ability to alter PM permeability; the mutant Y170A, however, could pass through the PM, but degraded in the space between the PM and the midgut epithelium. Further characterization by oligomerization demonstrated that Arg-158 mutants failed to form correctly sized high-molecular weight oligomers. This is the first report that Arg-158 plays a role in the formation of Cry4Ba oligomers, which are essential for toxin passage across the PM. Tyr-170, meanwhile, is involved in toxin stabilization in the toxic mechanism of Cry4Ba in mosquito larvae. [BMB Reports 2014; 47(10): 546-551]
ISSN: 1976-670X (electronic)
Appears in Collections:MB-Article

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