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|Title:||Development of simple sequence repeat markers from expressed sequence tags of the black tiger shrimp (Penaeus monodon)|
Thailand National Center for Genetic Engineering and Biotechnology
|Keywords:||Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology|
|Citation:||Molecular Ecology Resources. Vol.8, No.6 (2008), 1494-1496|
|Abstract:||In this study, we describe the development of expressed sequence tag-simple sequence repeat markers from expressed sequence tags of the black tiger shrimp (Penaeus monodon) deposited in public sequence databases. A total of 46 primer pairs were designed and screened on 26 individuals of P. monodon from a natural population. Of these, 16 primer pairs showed polymorphic profiles with between two and five alleles per locus. The average unbiased and direct count heterozygosities were 0.4662 and 0.3516, respectively. Cross-amplification was tested with five individuals of Penaeus vannamei and polymorphic products were detected at five loci. © 2008 The Authors.|
|Appears in Collections:||Scopus 2006-2010|
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