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Title: Leptospira wolffii sp. nov., isolated from a human with suspected leptospirosis in Thailand
Authors: Andrew T. Slack
Thareerat Kalambaheti
Meegan L. Symonds
Michael F. Dohnt
Renee L. Galloway
Arnold G. Steigerwalt
Wanpen Chaicumpa
Gaysorn Bunyaraksyotin
Scott Craig
Bruce J. Harrower
Lee D. Smythe
World Health Organization, Australia
Mahidol University
Coordinating Center for Infectious Diseases
Thammasat University
Thailand Ministry of Public Health
Queensland Health
Keywords: Agricultural and Biological Sciences;Immunology and Microbiology
Issue Date: 1-Oct-2008
Citation: International Journal of Systematic and Evolutionary Microbiology. Vol.58, No.10 (2008), 2305-2308
Abstract: A single Leptospira strain (designated Khorat-H2T) was isolated from the urine of an adult male patient with suspected leptospirosis from the province of Nakornrachasima, Thailand. The isolate showed typical Leptospira motility and morphology under dark-field microscopy. Cells were 10-13 μm long and 0.2 μm in diameter, with a wavelength of 0.5 μm and an amplitude of approximately 0.3 μm. Phenotypically, strain Khorat-H2T did not grow at 13 °C but grew at 30 and 37 °C and in the presence of 8-azaguanine. Serological identification using the microscopic agglutination test revealed that strain Khorat-H2T had no cross-reaction with any recognized Leptospira serogroups. Phylogenetic analysis of the 16S rRNA gene sequence placed the novel strain within the radiation of the genus Leptospira, with sequence similarities of 88.1-97.7% to recognized Leptospira species. DNA-DNA hybridization against the type strains of the three most closely related Leptospira species was used to confirm the results of the 16S rRNA sequence analysis. The G+C content of strain Khorat-H2T was 41.8 mol%. On the basis of phenotypic, serological and phylogenetic data, strain Khorat-H2T represents a novel species of the genus Leptospira, for which the name Leptospira wolffii sp. nov. is proposed. The type strain is Khorat-H2T (=WHO LT1686T =KIT Khorat-H2T). © 2008 IUMS.
ISSN: 14665026
Appears in Collections:Scopus 2006-2010

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