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Title: Morphological and biochemical alterations of abalone testicular germ cells and spawned sperm and their fertilizing ability
Authors: Worawit Suphamungmee
Wattana Weerachatyanukul
Tanes Poomtong
Peter Hanna
Prasert Sobhon
Mahidol University
The Coastal Fisheries Research and Development Center
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Sep-2008
Citation: Marine Biotechnology. Vol.10, No.5 (2008), 593-601
Abstract: In this study, we aimed to detect morphological and biochemical changes in developing germ cells (Gc), testicular sperm (Tsp), and spawned sperm (Ssp) using capacitation-associated characteristics. Gradual changes in the profiles of two membrane proteins, namely NaCl- and detergent-extractable proteins, were observed as compared Gc with Tsp and Tsp with Ssp. These membrane modifications were accomplished mostly through the introduction of new protein sets, both peripheral and integral, into Tsp and Ssp membranes. Activation of serine proteases, particularly in Ssp detergent-extracted proteins with the molecular masses of 38-130 kDa was evident and marked a major difference between Ssp and Tsp. An increase in the level of tyrosine phosphorylation of the proteins ranging from 15 to 20 kDa was noted in Tsp and remained constant in Ssp. Specifically, these three capacitation-associated characteristics could be detected in Ssp, possessing full fertilizing capacity. The lack of an activated proteolytic activity in Tsp resulted in a delayed fertilization, but not affected fertilizing ability. We believe that these characteristics should be advantageous in predicting abalone sperm fertilizing capability, particularly in cases when isolated germ cells or purified Tsp are used in place of spawned sperm in abalone aquaculture. © 2008 Springer Science+Business Media, LLC.
ISSN: 14362228
Appears in Collections:Scopus 2006-2010

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