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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/18722
Title: Antimicrobial activity of a protein purified from the latex of Hevea brasiliensis on oral microorganisms
Authors: Kanyanatt Kanokwiroon
Rawee Teanpaisan
Dhirayos Wititsuwannakul
Alan B. Hooper
Rapepun Wititsuwannakul
Prince of Songkla University
Mahidol University
University of Minnesota System
Keywords: Agricultural and Biological Sciences;Immunology and Microbiology;Medicine
Issue Date: 1-Jul-2008
Citation: Mycoses. Vol.51, No.4 (2008), 301-307
Abstract: This study aimed at screen for antimicrobial activity present in the non-rubber constituents of rubber latex of Hevea brasiliensis against various microbial strains. An antimicrobial protein, hevein was extracted from the bottom fraction after centrifugation and purified by acetone fractionation and anion exchange chromatography on a DEAE-Sepharose Fast Flow column. This procedure was more efficient and rapid than the previously described procedures. The antimicrobial activity was investigated and revealed that hevein, a small (4.7 kDa) cysteine-rich protein, had strong antimicrobial activity, especially against Candida spp. including Candida albicans, Candida tropicalis and Candida krusei. The MIC80 value for hevein was as low as 12 μg ml -1 with C. tropicalis ATCC 750. Higher MIC80 values were obtained for C. albicans ATCC 10231 (95 μg ml-1) and C. krusei ATCC 6258 (190 μg ml-1). To confirm the antifungal activity, hevein also inhibited the growth of those fungi in a disk diffusion assay and its inhibition was enhanced when a Hevea latex protease inhibitor was also included. Hevein at a concentration of 30 μg ml-1 also caused a Ca2+-dependent aggregation of C. tropicalis yeast cells. These data indicate that hevein can inhibit the growth of certain potential oral fungal pathogens. © 2008 The Authors.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=44849091840&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/18722
ISSN: 14390507
09337407
Appears in Collections:Scopus 2006-2010

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