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Title: Vanadium-dependent bromoperoxidases from Gracilaria algae
Authors: Tuangporn Suthiphongchai
Patcharee Boonsiri
Bhinyo Panijpan
Mahidol University
Khon Kaen University
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Jun-2008
Citation: Journal of Applied Phycology. Vol.20, No.3 (2008), 271-278
Abstract: Red algae from the Gulf of Thailand were examined for haloperoxidatic activity. Six species, Gracilaria changii, G. edulis, G. firma, G. fisheri, G. salicornia, and G. tenuistipitata, showed bromoperoxidatic activity. Duplicate polyacrylamide electrophoretic gels showed enzyme activity patterns developed by phenol red staining for bromoperoxidatic activity and by 3,3′- diaminobenzidine staining for peroxidatic activity. All algae gave isoenzymic bromoperoxidatic activity bands and peroxidatic activity bands, but there were peroxidatic and bromoperoxidatic activity bands that did not correspond. The bromoperoxidatic activity of the crude enzyme extracts as well as previously dialyzed enzyme solutions was enhanced significantly by incubation with vanadium pentoxide. The three purified bromoperoxidases from G. fisheri contained vanadium, and their relative activities corresponded to the ratio of vanadium to enzyme. In addition, they were not inhibited by H2O2. These data confirm that the enzymes are vanadium bromoperoxidases. © 2007 Springer Science+Business Media B.V.
ISSN: 09218971
Appears in Collections:Scopus 2006-2010

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