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Title: The presence of GABA in gastropod mucus and its role in inducing larval settlement
Authors: Praphaporn Laimek
Shona Clark
Michael Stewart
Fred Pfeffer
Chaitip Wanichanon
Peter Hanna
Prasert Sobhon
Mahidol University
Deakin University
Keywords: Agricultural and Biological Sciences
Issue Date: 18-Jan-2008
Citation: Journal of Experimental Marine Biology and Ecology. Vol.354, No.2 (2008), 182-191
Abstract: Chemical substances that induce larval settlement have been the focus of many gastropod studies due to the importance of wild stock recruitment and production within aquaculture facilities. Gamma-aminobutyric acid (GABA), GABA analogs, and GABA-mimetics associated with certain crustose coralline algae (CCA), are known to induce larval settlement in commercial abalone (Haliotis) species, and other gastropods. Furthermore, mucus secreted from these gastropods has been shown to induce larval settlement, but the stimulatory components of mucus have not been thoroughly investigated. We now present data confirming that GABA is the settlement-inducing effector molecule contained within abalone mucus. To do this, we initially generated anti-GABA for use in immunoenzyme and immunofluorescent microscopy. Using these techniques GABA was identified in the nerves and epithelial cells of the foot, including mucus. Dried mucus samples subject to HPLC analysis revealed a mean concentration of 0.68 mM GABA after sample rehydration. The presence of GABA in these samples was confirmed by time-of-flight mass spectroscopy (TOF-MS). In addition, GABA was detected in the mucus of several abalone species and other gastropods by immunocytochemistry. Subsequent bioassays using both dry and fresh mucus strongly promoted induction of larval settlement. © 2007 Elsevier B.V. All rights reserved.
ISSN: 00220981
Appears in Collections:Scopus 2006-2010

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