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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/18827
Title: Genomic islands from five strains of Burkholderia pseudomallei
Authors: Apichai Tuanyok
Benjamin R. Leadem
Raymond K. Auerbach
Stephen M. Beckstrom-Sternberg
James S. Beckstrom-Sternberg
Mark Mayo
Vanaporn Wuthiekanun
Thomas S. Brettin
William C. Nierman
Sharon J. Peacock
Bart J. Currie
David M. Wagner
Paul Keim
Northern Arizona University
Translational Genomics Research Institute
Menzies School of Health Research
Mahidol University
Los Alamos National Laboratory
J. Craig Venter Institute
George Washington University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 27-Nov-2008
Citation: BMC Genomics. Vol.9, (2008)
Abstract: Background: Burkholderia pseudomallei is the etiologic agent of melioidosis, a significant cause of morbidity and mortality where this infection is endemic. Genomic differences among strains of B. pseudomallei are predicted to be one of the major causes of the diverse clinical manifestations observed among patients with melioidosis. The purpose of this study was to examine the role of genomic islands (GIs) as sources of genomic diversity in this species. Results: We found that genomic islands (GIs) vary greatly among B. pseudomallei strains. We identified 71 distinct GIs from the genome sequences of five reference strains of B. pseudomallei: K96243, 1710b, 1106a, MSHR668, and MSHR305. The genomic positions of these GIs are not random, as many of them are associated with tRNA gene loci. In particular, the 3′ end sequences of tRNA genes are predicted to be involved in the integration of GIs. We propose the term "tRNA-mediated site-specific recombination" (tRNA-SSR) for this mechanism. In addition, we provide a GI nomenclature that is based upon integration hotspots identified here or previously described. Conclusion: Our data suggest that acquisition of GIs is one of the major sources of genomic diversity within B. pseudomallei and the molecular mechanisms that facilitate horizontally-acquired GIs are common across multiple strains of B. pseudomallei. The differential presence of the 71 GIs across multiple strains demonstrates the importance of these mobile elements for shaping the genetic composition of individual strains and populations within this bacterial species. © 2008 Tuanyok et al; licensee BioMed Central Ltd.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=58149313638&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/18827
ISSN: 14712164
Appears in Collections:Scopus 2006-2010

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