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Title: Application of carbohydrate microarray technology for the detection of Burkholderia pseudomallei, Bacillus anthracis and Francisella tularensis antibodies
Authors: N. Parthasarathy
R. Saksena
P. Kováč
D. DeShazer
S. J. Peacock
V. Wuthiekanun
H. S. Heine
A. M. Friedlander
C. K. Cote
S. L. Welkos
J. J. Adamovicz
S. Bavari
D. M. Waag
U.S. Army Medical Research Institute of Infectious Diseases
National Institute of Diabetes and Digestive and Kidney Diseases
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry
Issue Date: 3-Nov-2008
Citation: Carbohydrate Research. Vol.343, No.16 (2008), 2783-2788
Abstract: We developed a microarray platform by immobilizing bacterial 'signature' carbohydrates onto epoxide modified glass slides. The carbohydrate microarray platform was probed with sera from non-melioidosis and melioidosis (Burkholderia pseudomallei) individuals. The platform was also probed with sera from rabbits vaccinated with Bacillus anthracis spores and Francisella tularensis bacteria. By employing this microarray platform, we were able to detect and differentiate B. pseudomallei, B. anthracis and F. tularensis antibodies in infected patients, and infected or vaccinated animals. These antibodies were absent in the sera of nai{dotless}̈ve test subjects. The advantages of the carbohydrate microarray technology over the traditional indirect hemagglutination and microagglutination tests for the serodiagnosis of melioidosis and tularemia are discussed. Furthermore, this array is a multiplex carbohydrate microarray for the detection of all three biothreat bacterial infections including melioidosis, anthrax and tularemia with one, multivalent device. The implication is that this technology could be expanded to include a wide array of infectious and biothreat agents. © 2008 Elsevier Ltd.
ISSN: 00086215
Appears in Collections:Scopus 2006-2010

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