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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/18836
Title: Isolation and characterization of bamA genes, homologues of the γ-butyrolactone autoregulator-receptor gene in Amycolatopsis mediterranei, a rifamycin producer
Authors: Aiyada Aroonsri
Shigeru Kitani
Sun Uk Choi
Takuya Nihira
Osaka University
Kyungnam University
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Nov-2008
Citation: Biotechnology Letters. Vol.30, No.11 (2008), 2019-2024
Abstract: Four genes (bamA1, bamA2, bamA3 and bamA4) encoding homologues of the γ-butyrolactone autoregulator receptor of Streptomyces were found and cloned from Amycolatopsis mediterranei, a typical non-Streptomyces actinomycetes and a producer of rifamycin, one of the major anti-tuberculosis drugs in clinical treatment. Transcriptional analysis demonstrated that bamA1 and bamA2 are transcribed in a growth-dependent manner, while bamA3 and bamA4 are constitutively transcribed during growth. Binding assays using3H-labeled autoregulator analogues as ligands confirmed that all of the recombinant BamA proteins expressed in Escherichia coli have clear binding activity toward several types of Streptomyces autoregulators. The ligand specificity of the recombinant BamA1 protein was identical to that of the crude cell-free lysates of A. mediterranei reported in our previous work. These results suggest that A. mediterranei, which is phylogenetically situated in a distal clade from the genus Streptomyces as non-Streptomyces actinomycetes, has an autoregulator-mediated signaling system. © 2008 Springer Science+Business Media B.V.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=52449110315&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/18836
ISSN: 15736776
01415492
Appears in Collections:Scopus 2006-2010

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