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dc.contributor.authorVisith Thongboonkerden_US
dc.contributor.authorTheptida Semangoenen_US
dc.contributor.authorSupachok Sinchaikulen_US
dc.contributor.authorShui Tein Chenen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherGenomics Research Center, Academia Sinicaen_US
dc.contributor.otherNational Taiwan Universityen_US
dc.contributor.otherFaculty of Medicine, Siriraj Hospital, Mahidol Universityen_US
dc.identifier.citationJournal of Proteome Research. Vol.7, No.11 (2008), 4689-4700en_US
dc.description.abstractCalcium oxalate monohydrate (COM) is the major crystalline component found in kidney stones and its adhesion to renal tubular cells provokes tubular injury, which in turn enhances COM crystal adhesion. However, COM-induced toxic effects in these tubular cells remain largely unknown. We performed a proteomics study to characterize changes in the cellular proteome in MDCK distal renal tubular cells after an exposure to high-dose (1000 μg/mL) COM crystals for 48 h, at which percentage of cell death was significantly increased. Proteins were extracted from MDCK cells cultured with COM-containing or COM-free medium (n = 5 individual flasks per group), resolved in individual 2-D gels, and stained with SYPRO Ruby fluorescence dye. Quantitative and statistical analyses revealed 53 proteins whose abundance levels were altered (25 were increased, whereas other 28 were decreased) by COM-induced toxicity. Among these, 50 were successfully identified by quadrupole time-of-flight (Q-TOF) mass spectrometry (MS) and/or tandem MS (MS/MS) analyses. The proteomic data were clearly confirmed by 2-D Western blot analysis. While three chaperones (GRP78, Orp150 and Hsp60) were increased, other proteins involved in protein biosynthesis, ATP synthesis, cell cycle regulator, cellular structure, and signal transduction were decreased. These data provide some novel mechanistic insights into the molecular mechanisms of COM crystal-induced tubular toxicity. © 2008 American Chemical Society.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleProteomic analysis of calcium oxalate monohydrate crystal-induced cytotoxicity in distal renal tubular cellsen_US
Appears in Collections:Scopus 2006-2010

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