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Title: The core and accessory genomes of Burkholderia pseudomallei: Implications for human melioidosis
Authors: Hoon Sim Siew
Yiting Yu
Ho Lin Chi
R. Krishna M. Karuturi
Vanaporn Wuthiekanun
Apichai Tuanyok
Hoon Chua Hui
Catherine Ong
Sivalingam Suppiah Paramalingam
Gladys Tan
Lynn Tang
Gary Lau
Eong Ooi Eng
Donald Woods
Edward Feil
Sharon J. Peacock
Patrick Tan
DSO National Laboratories
Genome Institute of Singapore
Mahidol University
Health Sciences Centre Calgary
University of Bath
Nuffield Department of Clinical Medicine
Duke-NUS Graduate Medical School Singapore
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Oct-2008
Citation: PLoS Pathogens. Vol.4, No.10 (2008)
Abstract: Natural isolates of Burkholderia pseudomallei (Bp), the causative agent of melioidosis, can exhibit significant ecological flexibility that is likely reflective of a dynamic genome. Using whole-genome Bp microarrays, we examined patterns of gene presence and absence across 94 South East Asian strains isolated from a variety of clinical, environmental, or animal sources. 86% of the Bp K96243 reference genome was common to all the strains representing the Bp "core genome", comprising genes largely involved in essential functions (eg amino acid metabolism, protein translation). In contrast, 14% of the K96243 genome was variably present across the isolates. This Bp accessory genome encompassed multiple genomic islands (GIs), paralogous genes, and insertions/deletions, including three distinct lipopolysaccharide (LPS)-related gene clusters. Strikingly, strains recovered from cases of human melioidosis clustered on a tree based on accessory gene content, and were significantly more likely to harbor certain GIs compared to animal and environmental isolates. Consistent with the inference that the GIs may contribute to pathogenesis, experimental mutation of BPSS2053, a GI gene, reduced microbial adherence to human epithelial cells. Our results suggest that the Bp accessory genome is likely to play an important role in microbial adaptation and virulence. © 2008 Sim et al.
ISSN: 15537374
Appears in Collections:Scopus 2006-2010

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