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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/18900
Title: The rpoE operon regulates heat stress response in Burkholderia pseudomallei
Authors: Muthita Vanaporn
Paiboon Vattanaviboon
Visith Thongboonkerd
Sunee Korbsrisate
Mahidol University
Chulabhorn Research Institute
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Jul-2008
Citation: FEMS Microbiology Letters. Vol.284, No.2 (2008), 191-196
Abstract: Burkholderia pseudomallei is a gram-negative bacterium and the causative agent of melioidosis, one of the important lethal diseases in tropical regions. In this article, we demonstrate the crucial role of the B. pseudomallei rpoE locus in the response to heat stress. The rpoE operon knockout mutant exhibited growth retardation and reduced survival when exposed to a high temperature. Expression analysis using rpoH promoter-lacZ fusion revealed that heat stress induction of rpoH, which encodes heat shock sigma factor (σH), was abolished in the B. pseudomallei rpoE mutant. Analysis of the rpoH promoter region revealed sequences sharing high homology to the consensus sequence of σE-dependent promoters. Moreover, the putative heat-induced σH-regulated heat shock proteins (i.e. GroEL and HtpG) were also absent in the rpoE operon mutant. Altogether, our data suggest that the rpoE operon regulates B. pseudomallei heat stress response through the function of rpoH. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=44949254948&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/18900
ISSN: 15746968
03781097
Appears in Collections:Scopus 2006-2010

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