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|Title:||The rpoE operon regulates heat stress response in Burkholderia pseudomallei|
Chulabhorn Research Institute
|Keywords:||Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology|
|Citation:||FEMS Microbiology Letters. Vol.284, No.2 (2008), 191-196|
|Abstract:||Burkholderia pseudomallei is a gram-negative bacterium and the causative agent of melioidosis, one of the important lethal diseases in tropical regions. In this article, we demonstrate the crucial role of the B. pseudomallei rpoE locus in the response to heat stress. The rpoE operon knockout mutant exhibited growth retardation and reduced survival when exposed to a high temperature. Expression analysis using rpoH promoter-lacZ fusion revealed that heat stress induction of rpoH, which encodes heat shock sigma factor (σH), was abolished in the B. pseudomallei rpoE mutant. Analysis of the rpoH promoter region revealed sequences sharing high homology to the consensus sequence of σE-dependent promoters. Moreover, the putative heat-induced σH-regulated heat shock proteins (i.e. GroEL and HtpG) were also absent in the rpoE operon mutant. Altogether, our data suggest that the rpoE operon regulates B. pseudomallei heat stress response through the function of rpoH. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.|
|Appears in Collections:||Scopus 2006-2010|
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