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|Title:||Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms|
|Authors:||Mark R. Liles|
Lynn L. Williamson
Robert M. Goodman
University of Wisconsin Madison
Universitetet i Bergen
School of Environmental and Biological Sciences
Rutgers, The State University of New Jersey
|Keywords:||Biochemistry, Genetics and Molecular Biology;Environmental Science;Immunology and Microbiology|
|Citation:||Applied and Environmental Microbiology. Vol.74, No.10 (2008), 3302-3305|
|Abstract:||We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic libraries. Copyright © 2008, American Society for Microbiology. All Rights Reserved.|
|Appears in Collections:||Scopus 2006-2010|
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