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Title: Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms
Authors: Mark R. Liles
Lynn L. Williamson
Jitsupang Rodbumrer
Vigdis Torsvik
Robert M. Goodman
Jo Handelsman
Auburn University
University of Wisconsin Madison
Mahidol University
Universitetet i Bergen
School of Environmental and Biological Sciences
Rutgers, The State University of New Jersey
Keywords: Biochemistry, Genetics and Molecular Biology;Environmental Science;Immunology and Microbiology
Issue Date: 1-May-2008
Citation: Applied and Environmental Microbiology. Vol.74, No.10 (2008), 3302-3305
Abstract: We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic libraries. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
ISSN: 00992240
Appears in Collections:Scopus 2006-2010

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