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Title: New BD FACSCount™ CD4 reagent system for simultaneous enumeration of percent and absolute CD4 T-lymphocytes in HIV-1-infected pediatric patients
Authors: Kovit Pattanapanyasat
Kasama Sukapirom
Laddawan Kowawisatsut
Charin Thepthai
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 28-Apr-2008
Citation: Cytometry Part B - Clinical Cytometry. Vol.74, No.SUPPL. 1 (2008)
Abstract: Background: Absolute CD4+ T-lymphocyte counts are used in the initiation and monitoring of antiretroviral therapy in HIV-infected patients. Becton Dickinson's (BD) FACSCount™ system was introduced 12 years ago as a dedicated instrument for enumeration of absolute CD4+ T-lymphocytes. However, this system does not provide percent CD4+ T-lymphocyte that is the required monitoring parameter in pediatric patients. We evaluated a new BD FACSCount CD4 software and reagents for simultaneous percent and absolute CD4+ T-lymphocytes in HIV-infected blood. Methods: Percent and absolute CD4+ T-lymphocytes in 149 HIV-infected blood samples were determined using a new FACSCount system. Results of percent and absolute CD4+ T-lymphocytes were compared between the dual-platform (DP) method, using BD FACScan™ flow cytometer plus hematology analyzer and the standard FACSCount system. Correlation and agreement were analyzed using linear regression and Bland-Altman analysis. Results: Percent CD4+ T-lymphocyte values obtained from the new FACSCount system correlated well with DP FACScan method (r2= 0.977, P < 0.0001). Mean bias was only -0.36% [limit of agreement (LOA): -2.52% to +1.80%] and percent similarity was 101.36%. For absolute CD4+ T-lymphocyte, the new system correlated highly with standard FACSCount system (r2= 0.986, P < 0.0001), with a percent similarity of 98.2. Mean bias was +3.39 cells/μl with LOA of -52.53 cells/μ to +59.31 cells/μl. Conclusion: This new FACSCount system is a simple and reliable system for enumeration of absolute and percent CD4+ T-lymphocytes. Having one system giving both results should reduce the cost and thus increase access to CD4 testing for pediatric and adult patients. © 2008 Clinical Cytometry Society.
ISSN: 15524957
Appears in Collections:Scopus 2006-2010

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