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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/18947
Title: Characterization of Argonaute cDNA from Penaeus monodon and implication of its role in RNA interference
Authors: Manasave Dechklar
Apinunt Udomkit
Sakol Panyim
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 21-Mar-2008
Citation: Biochemical and Biophysical Research Communications. Vol.367, No.4 (2008), 768-774
Abstract: RNA interference (RNAi) has recently become a promising strategy for therapeutic of several viral diseases including those in the black tiger shrimp Penaeus monodon. However, the protein components that play role in RNAi in P. monodon have not yet been identified. Here, we report the cloning and functional characterization of a cDNA encoding Argonaute, a principal constituent of RNAi pathway in P. monodon. P. monodon's Argonaute (Pem-AGO) exhibited the two signature domains, PAZ and PIWI. Substantial level of Pem-ago expression could be suppressed by double-stranded RNA (dsRNA) that targeted PAZ coding sequence in shrimp primary culture of Oka cells. The Pem-ago depleted cells showed impaired RNAi as the expression of an endogenous gene was rescued from the dsRNA-mediated silencing in these cells. Our results imply that Pem-ago is required for effective RNAi in P. monodon and thus identify the first protein constituent of RNAi machinery in penaeid shrimp. © 2008 Elsevier Inc. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=38649089779&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/18947
ISSN: 10902104
0006291X
Appears in Collections:Scopus 2006-2010

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