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Title: Mosquito cells accommodate balanced, persistent co-infections with a densovirus and Dengue virus
Authors: Nipaporn Kanthong
Nuanpan Khemnu
Siriporn Sriurairatana
Sa Nga Pattanakitsakul
Prida Malasit
Timothy W. Flegel
Mahidol University
Faculty of Medicine, Siriraj Hospital, Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 17-Mar-2008
Citation: Developmental and Comparative Immunology. Vol.32, No.9 (2008), 1063-1075
Abstract: To study persistent viral co-infections in arthropods, we first produced stable, persistently infected C6/36 mosquito cell cultures by serial passage of exponentially growing whole cells infected with either a densovirus (AalDNV) or Dengue virus (DEN-2). We then obtained stable, persistent co-infections by reciprocal super-challenge and similar passaging. Persistently infected cultures did not differ from naïve-cell cultures in growth rate and cell morphology. Nor did they differ in high production of both viruses with high infection rates for naïve C6/36 cells. Immunocytochemistry revealed that 99-100% of the cells were coinfected but that super-infection order had some effect on antigen distribution for the two viruses. Our results combined with existing field information and previously published experimental work suggest that the capacity to support stable, viral co-infections may be a general phenomenon for arthropod cells, and that they may be achieved easily and rapidly by serial passaging of whole cultured cells. Such persistent infections would facilitate studies on interactions between co-infecting viruses. © 2008 Elsevier Ltd. All rights reserved.
ISSN: 0145305X
Appears in Collections:Scopus 2006-2010

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