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Title: A dual activation mechanism for Myb-responsive genes in myelomonocytic cells
Authors: Vichanan Yamkamon
Olga Ivanova
Daniel Braas
Olesya Chayka
Pimpicha Patmasiriwat
Karl Heinz Klempnauer
Westfalische Wilhelms-Universitat Munster
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 1-Mar-2008
Citation: Blood Cells, Molecules, and Diseases. Vol.40, No.2 (2008), 219-226
Abstract: The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which is responsible for the transformation of myelomonocytic cells by avian myeloblastosis virus (AMV). v-Myb is thought to exert its biological effects by deregulating the expression of specific target genes. Here we have used DNaseI hypersensitive site mapping and reporter gene assays to study the activation of three Myb target genes - mim-1, the lysozyme gene and the C/EBPβ gene - all of which are activated by Myb in myelomonocytic cells but not in other hematopoietic lineages. We have found that these genes are activated by Myb via more than one cis-regulatory region. Our data suggest that all three genes are activated by Myb by dual mechanisms involving the promoters as well as enhancers. Using a cell line that expresses an estrogen-inducible v-Myb/estrogen receptor fusion protein we have also determined the effect of Myb on the expression of the C/EBPα gene. Our results show that C/EBPα expression is down-regulated by v-Myb. Thus, v-Myb affects the expression of two C/EBP family members in opposite directions. © 2007 Elsevier Inc. All rights reserved.
ISSN: 10799796
Appears in Collections:Scopus 2006-2010

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