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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/19268
Title: Monoclonal antibodies to LipL32 protect against heterologous Leptospira spp. challenge
Authors: Santi Maneewatch
Yuwaporn Sakolvaree
Patcharin Saengjaruk
Potjanee Srimanote
Pramuan Tapchaisri
Pongsri Tongtawe
Buppa Klaysing
Surasakdi Wongratanacheewin
Manas Chongsa-Nguan
Wanpen Chaicumpa
Thammasat University
Mahidol University
Faculty of Medicine, Thammasat University
Khon Kaen University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Dec-2008
Citation: Hybridoma. Vol.27, No.6 (2008), 453-465
Abstract: A non-culture-based leptospirosis vaccine that cross-protects against infection caused by heterologous Leptospira spp. should replace the currently available products, which are qualitatively and quantitatively inadequate. With that in mind, two murine hybridomas secreting monoclonal antibodies (MAb) binding only to homogenates of pathogenic Leptospira spp., and not of the saprophytic L. biflexa, serogroup Patoc, serovar Patoc, were produced. The MAbs of both clones neutralized Leptospira-mediated human red blood cell lysis in vitro and rescued hamsters from lethal infection with heterologous Leptospira spp. The orthologous Leptospira spp. protein carrying the MAb epitope(s) was identified by two-dimensional gel electrophoresis (2DE)-based proteomics and database search. The epitopes of the MAbs were located on the major outer membrane protein LipL32 of the pathogenic Leptospira spp. The MAbs in their humanized version are potential leptospirosis immunotherapeutics. They are also suitable as detection reagents in antigen-based assays for the rapid diagnosis of leptospirosis. Recombinant LipL32 is a good candidate for a broad spectrum, non-culture-based leptospirosis vaccine. © Copyright 2008, Mary Ann Liebert, Inc.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=58149123710&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/19268
ISSN: 15540014
Appears in Collections:Scopus 2006-2010

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