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Title: Double-stranded RNA adenosine deaminases enhance expression of human immunodeficiency virus type 1 proteins
Authors: Angsana Phuphuakrat
Romchat Kraiwong
Chompunuch Boonarkart
Darat Lauhakirti
Tun H. Lee
Prasert Auewarakul
Mahidol University
Harvard School of Public Health
Faculty of Medicine, Siriraj Hospital, Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Nov-2008
Citation: Journal of Virology. Vol.82, No.21 (2008), 10864-10872
Abstract: ADARs (adenosine deaminases that act on double-stranded RNA) are RNA editing enzymes that catalyze a change from adenosine to inosine, which is then recognized as guanosine by translational machinery. We demonstrate here that overexpression of ADARs but not of an ADAR mutant lacking editing activity could upregulate human immunodeficiency virus type 1 (HIV-1) structural protein expression and viral production. Knockdown of ADAR1 by RNA silencing inhibited HIV-1 production. Viral RNA harvested from transfected ADAR1-knocked-down cells showed a decrease in the level of unspliced RNA transcripts. Overexpression of ADAR1 induced editing at a specific site in the env gene, and a mutant with the edited sequence was expressed more efficiently than the wild-type viral genome. These data suggested the role of ADAR in modulation of HIV-1 replication. Our data demonstrate a novel mechanism in which HIV-1 employs host RNA modification machinery for posttranscriptional regulation of viral protein expression. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
ISSN: 0022538X
Appears in Collections:Scopus 2006-2010

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