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Title: LightCycler™ real-time PCR for rapid detection and quantitation of Mycobacterium leprae in skin specimens
Authors: Janisara Rudeeaneksin
Sopa Srisungngam
Pathom Sawanpanyalert
Thaverit Sittiwakin
Sirirat Likanonsakul
Supannee Pasadorn
Prasit Palittapongarnpim
Patrick J. Brennan
Benjawan Phetsuksiri
National Institutes of Health, Bethesda
Thailand Ministry of Public Health
Bamrasnaradura Infectious Disease Institute
Mahidol University
Colorado State University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Nov-2008
Citation: FEMS Immunology and Medical Microbiology. Vol.54, No.2 (2008), 263-270
Abstract: Diagnosis of leprosy is usually based on clinical features and skin smear results including the number of skin lesions. Mycobacterium leprae is not cultivable and bacterial enumeration by microscopic examination is required for leprosy classification, choice in choosing and monitoring chemotherapy regimens, and diagnosis of relapse. However, detection and quantification using standard microscopy yields results of limited specificity and sensitivity. We describe an extremely sensitive and specific assay for the detection and quantification of M. leprae in skin biopsy specimens. Primers that amplified a specific 171-bp fragment of M. leprae 16S rRNA gene were chosen and specificity was verified by amplicon melting temperature. The method is sensitive enough to detect as low as 20 fg of M. leprae DNA, equivalent to four bacilli. The assay showed 100% concordance with clinical diagnosis in cases of multibacillary patients, and 50% of paucibacillary leprosy. The entire procedure of DNA extraction and PCR could be performed in c. 3 h. According to normalized quantitative real-time PCR, the patients in this study had bacilli numbers in the range of 1.07 × 10 2-1.65 × 108 per 6-mm3 skin biopsy specimen. This simple real-time PCR assay is a facile tool with possible applications for rapid detection and simultaneous quantification of leprosy bacilli in clinical samples. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
ISSN: 1574695X
Appears in Collections:Scopus 2006-2010

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