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Title: Comparison of 5 flow cytometric immunophenotyping systems for absolute CD4<sup>+</sup> T-lymphocyte counts in HIV-1-infected patients living in resource-limited settings
Authors: Kovit Pattanapanyasat
Yuwadee Phuang-Ngern
Kasama Sukapirom
Surada Lerdwana
Charin Thepthai
Boonrat Tassaneetrithep
Mahidol University
Keywords: Medicine
Issue Date: 1-Dec-2008
Citation: Journal of Acquired Immune Deficiency Syndromes. Vol.49, No.4 (2008), 339-347
Abstract: Enumeration of CD4+ T lymphocytes is important in management of HIV-infected patients. However, CD4 testing by current gold standard bead-based flow cytometer (FCM) system is expensive for developing countries. This study compared 2 affordable volumetric FCMs with the 3 predicate FCM systems. CD4 + T-lymphocyte counts on blood samples from 150 HIV-1-infected Thai patients were determined in parallel by 5 FCM systems: the 2 single-platform volumetric FCM systems, Guava and CyFlowgreen; the 2 standard single-platform bead-based systems (2-color FACSCount and the TriTEST/TruCOUNT tube using a FACSCalibur FCM); and the dual-platform TriTEST system. Correlation and agreement were analyzed using linear regression and Bland-Altman analysis. Results from these 2 volumetric systems gave similar results and excellent correlation: R2 > 0.93; mean biases ranged from +6.3 to +24.1 cells per microliter more for the Guava. In contrast, the CyFlowgreen showed the lowest values with R2 > 0.97; mean biases ranged from -9.8 to -27.6 cells per microliter. This indicates that the absolute CD4 + T-lymphocyte counts determined by CyFlowgreen are <FACSCount<DP TriTEST< TriTEST/TruCOUNT<Guava. Although the use of these 2 volumetric FCMs could make CD4+ T-lymphocyte enumeration more affordable in resource-poor settings, variations among these systems should be considered if these are to be interchanged. Copyright © 2008 by Lippincott Williams & Wilkins.
ISSN: 15254135
Appears in Collections:Scopus 2006-2010

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