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Title: Characterization of 2-deoxy-D-glucose uptake in fibroblast cultures derived from patients with A3243G mitochondrial DNA mutation
Authors: Komon Luangtrakool
Farrah Yasmin Tate
Rachael Shepherd
Sarah Campbell
Carolyn M. Sue
Patcharee Lertrit
Mahidol University
Royal North Shore Hospital
Keywords: Medicine
Issue Date: 1-Jul-2008
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.39, No.4 (2008), 745-752
Abstract: We investigated cellular glucose uptake of fibroblast cultures derived from seven patients with mitochondrial DNA (mtDNA) A3243G mutation and from six healthy controls with no mtDNA mutations. Heteroplasmy of fibroblast cultures were shifted by culturing for 5 days in galactose-containing medium. The proportion of mutant mtDNA decreased by 7.7% to 10% in three patient fibroblast cultures, whereas 2-deoxy-D-glucose uptake increased 1.8-2.1 -fold at basal state, 1.9-2.3-fold in the presence of 60 ng/ml of insulin, and 1.8-2.1-fold in 100 ng/ ml of insulin. No significant changes in level of heteroplasmy or glucose uptake were observed in the other patients samples and control samples. This study showed that alteration in the proportion of fibroblast mtDNA A3243G mutation content directly affected basal and insulin-stimulated glucose uptake.
ISSN: 01251562
Appears in Collections:Scopus 2006-2010

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