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Title: Immune responses of selected phagotopes from monoclonal antibodies of Burkholderia pseudomallei
Authors: Narisorn Na-ngam
Thareerat Kalambaheti
Pattama Ekpo
Annamthip Pitaksajjakul
Natsuda Jamornthanyawat
Narisara Chantratita
Stitaya Sirisinha
Visanu Thamlikitkul
Wanpen Chaicumpa
Montarop Yamabhai
Pongrama Ramasoota
Khon Kaen University
Department of Microbiology and Immunology
Mahidol University
Suranaree University of Technology
Keywords: Medicine
Issue Date: 1-May-2008
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.39, No.3 (2008), 443-451
Abstract: Random peptide libraries displayed by bacteriophage T7 and M13 were employed to identify mimotopes from 4 monoclonal antibodies (MAbs) specific to Burkholderia pseudomallei. Insert DNA sequences of bound phages selected from four rounds of panning with each MAb revealed peptide sequences corresponding to B. pseudomallei K96243 hypothetical protein BPSL2046, hypothetical protein BpseP_02000035, B. pseudomallei K96243 hypothetical protein BPSS0784, B. pseudomallei 1710b hypothetical protein BURPS1710b_1104, and 6. cenocepacia H12424 TonB-dependent siderophore receptor, all located at the outer membrane. The immune responses from all selected phagotopes were significantly higher than that of lipopolysaccharide. The study demonstrates the feasibility of identifying mimotopes through screening of phage-displayed random peptide libraries with B. pseudomallei MAbs.
ISSN: 01251562
Appears in Collections:Scopus 2006-2010

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