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Title: Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos
Authors: Stuart D. Blacksell
Mammen P. Mammen
Soulignasack Thongpaseuth
Robert V. Gibbons
Richard G. Jarman
Kemajittra Jenjaroen
Ananda Nisalak
Rattanaphone Phetsouvanh
Paul N. Newton
Nicholas P.J. Day
Mahosot Hospital
Churchill Hospital
Mahidol University
Armed Forces Research Institute of Medical Sciences, Thailand
Keywords: Medicine
Issue Date: 1-Jan-2008
Citation: Diagnostic Microbiology and Infectious Disease. Vol.60, No.1 (2008), 43-49
Abstract: We evaluated 2 commercial enzyme-linked immunosorbent assays (ELISAs) for the diagnosis of dengue infection; one a serologic test for immunoglobulin M (IgM) antibodies, the other based on detection of dengue virus nonstructural 1 (NS1) antigen. Using gold standard reference serology on paired sera, 41% (38/92 patients) were dengue confirmed, with 4 (11%) acute primary and 33 (87%) acute secondary infections (1 was of indeterminate status). Sensitivity of the NS1-ELISA was 63% (95% confidence interval [CI], 53-73) on admission samples but was much less sensitive (5%; 95% CI, 1-10) on convalescent samples. The IgM capture ELISA had a lower but statistically equivalent sensitivity compared with the NS1-ELISA for admission samples (45%; 95% CI, 35-55) but was more sensitive on convalescent samples (58%; 95% CI, 48-68). The results of the NS1 and IgM capture ELISAs were combined using a logical OR operator, increasing the sensitivity for admission samples (79%; 95% CI, 71-87), convalescent samples (63%; 95% CI, 53-73), and all samples (71%; 95% CI, 65-78). © 2008 Elsevier Inc. All rights reserved.
ISSN: 07328893
Appears in Collections:Scopus 2006-2010

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