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Title: Development of an amperometric immunosensor for the determination of methamphetamine in urine
Authors: Kavinyanee Luangaram
Dolurdee Boonsua
Sarisak Soontornchai
Chamras Promptmas
Mahidol University
Sukhothai Thammatirat Open University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemical Engineering
Issue Date: 1-Dec-2002
Citation: Biocatalysis and Biotransformation. Vol.20, No.6 (2002), 397-403
Abstract: An amperometric immunosensor in the competitive format was developed for the detection of methamphetamine in urine. The electrodes consisted of carbon paste and Ag/AgCl screen printed on heat sealing film, respectively, and of monoclonal lanti-methamphetamine antibody as the biorecognition element. Optimum amounts of methamphetamine-N-bovine serum albumin conjugate, monoclonal antibody and alkaline phosphatase-goat anti-mouse immunoglobulin G were 20, 10 ng and 1:10,000 dilution in 10 μl each, respectively. Methamphetamine was detected by the conversion of p-aminophenyl phosphate to electroactive p-aminophenol in the range of 200 ng/ml (lower detection limit) to 1,500 ng/ml methamphetamine in a nearly linear dose response curve. Within amphetamine concentrations of 0-1,500 ng/ml cross-reaction with methamphethamine was not observed. Working with urine samples spiked with methamphetamine, the accuracy and precision of the assay were 91.5-104.4% and 15.8-24.4%, respectively. This is a proof of concept in the clinical perspective for an amperometric immunosensor whose electrodes are amenable to future mass production.
ISSN: 10242422
Appears in Collections:Scopus 2001-2005

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