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Title: In-vitro cultivation: A sensitive method for detecting Blastocystis hominis
Authors: S. Leelayoova
P. Taamasri
R. Rangsin
T. Naaglor
U. Thathaisong
M. Mungthin
Phramongkutklao College of Medicine
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Dec-2002
Citation: Annals of Tropical Medicine and Parasitology. Vol.96, No.8 (2002), 803-807
Abstract: Currently, the detection of human infection with Blastocystis hominis is usually based on the examination under a light microscope of faecal samples, either directly, as 'simple smears', or after some form of concentration. Whether short-term, in-vitro cultivation would increase the sensitivity of such detection remains a matter of controversy. Over 900 fresh stool specimens, from soldiers in the Royal Thai Army, were each checked for the parasite using three methods: simple smears; formalin-ethyl-acetate concentration; and cultivation in Jones' medium. Although 334 of the samples were found to be culture-positive, the parasites were only detected in 142 of the simple smears, and faecal concentration led to an even lower sensitivity (64 positive samples). In-vitro cultivation does seem worthwhile in the detection of B. hominis carriage in field studies.
ISSN: 00034983
Appears in Collections:Scopus 2001-2005

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