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dc.contributor.authorA. Sirisriroen_US
dc.contributor.authorR. Gramsen_US
dc.contributor.authorS. Vichasri-Gramsen_US
dc.contributor.authorP. Ardseungneonen_US
dc.contributor.authorV. Pankaoen_US
dc.contributor.authorA. Meepoolen_US
dc.contributor.authorK. Chaithirayanonen_US
dc.contributor.authorV. Viyananten_US
dc.contributor.authorP. Tan-Ariyaen_US
dc.contributor.authorE. S. Upathamen_US
dc.contributor.authorP. Sobhonen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherThammasat Universityen_US
dc.date.accessioned2018-07-24T03:01:00Z-
dc.date.available2018-07-24T03:01:00Z-
dc.date.issued2002-04-30en_US
dc.identifier.citationVeterinary Parasitology. Vol.105, No.2 (2002), 119-129en_US
dc.identifier.issn03044017en_US
dc.identifier.other2-s2.0-18344362612en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=18344362612&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/20212-
dc.description.abstractIn Fasciola parasites fatty acid binding proteins (FABPs) are the carrier proteins that help in the uptake of fatty acids from the hosts' fluids. Attempts have been made to utilize both native and recombinant FABP (rFABP) for immunodiagnosis and vaccine development for fasciolosis. In this study, we have produced a number of monoclonal antibodies (MoAbs) against rFABP of Fasciola gigantica. These MoAbs were initially screened against rFABP by ELISA and then tested for their specificities by immunoblotting. Five stable clones were selected and characterized further: four of them were of the isotype IgG1while one clone was IgG2a. All the MoAbs reacted with rFABP which has a molecular weight (MW) of 20 kD and with at least two isoforms of native proteins at MW 14.5 kD that were present in the tegumental antigen (TA) and crude worm extracts, and the excretion-secretion materials. Immunoperoxidase staining of frozen sections of adult parasites by using these MoAbs as primary antibodies indicated that FABP were present in high concentration in the parenchymal cells and reproductive tissues, in low concentration in the tegument and caecal epithelium. All MoAbs cross-reacted with a 14.5 kD antigen present in the whole body (WB) extract of Schistosoma mansoni, while no cross-reactivities were detected with antigens from Eurytrema pancreaticum and Paramphistomum spp. © 2002 Elsevier Science B.V. All rights reserved.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=18344362612&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectVeterinaryen_US
dc.titleProduction and characterization of a monoclonal antibody against recombinant fatty acid binding protein of Fasciola giganticaen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1016/S0304-4017(02)00007-9en_US
Appears in Collections:Scopus 2001-2005

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