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Title: Flow cytometry for the analysis of T cells expressing CD69 after stimulation with glutamic acid decarboxylase
Authors: Watip Boonyasrisawat
Napatawn Banchuin
Kovit Pattanapanyasat
Chaicharn Deerochanawong
Pa Thai Yenchitsomanus
Sirirat Ploybutr
Sathit Vannasaeng
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jan-2002
Citation: Asian Pacific Journal of Allergy and Immunology. Vol.20, No.1 (2002), 37-42
Abstract: Type 1 diabetes mellitus is a T-cell mediated autoimmune disease in which the insulin-producing pancreatic beta cells are selectively destroyed. We recently found that the detection of cell-mediated immune response to glutamic acid decarboxylase (GAD) was more useful than the detection of specific autoantibodies for the diagnosis of type 1 diabetes mellitus. In this study, we established a flow cytometric analysis for the detection of activated T cells in whole venous blood, obtained from diabetic patients and normal controls after stimulation by GAD. Two millitiers of peripheral venous blood and 6 hours incubation time were used for performing the test. It was found that 33% (3/9) type 1 diabetic patients, 7.7% (1/13) type 2 diabetic patients and neither patients with fibrocalculous pancreatopathy nor normal controls had ≥ 20% CD8+ T cells expressing CD69. The results suggest that flow cytometry may be a useful tool for the detection of surrogate markers of type 1 diabetes mellitus.
ISSN: 0125877X
Appears in Collections:Scopus 2001-2005

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