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Title: Use of microsatellite variation and RAPD-PCR to assess genetic polymorphism in Biomphalaria glabrata snails from a single locale in a schistosomiasis endemic area
Authors: Christopher Rowe
Wannaporn Ittiprasert
Carolyn Patterson
Claudia Eliff
Kristen Page
Susan Bandoni
Thomas Wilke
Dennis Minchella
Fred Lewis
Matty Knight
Biomedical Research Institute, Rockville
Mahidol University
Wheaton College - Illinois
State University of New York Geneseo
George Washington University
Purdue University
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Dec-2003
Citation: Malacologia. Vol.45, No.1 (2003), 149-166
Abstract: Genetic variation was assessed in Biomphalaria glabrata snails using variations in microsatellite loci and by RAPD-PCR analysis. Populations of snails examined were field-collected isolates from a small pond in a schistosomiasis endemic region in Brazil, after standard conditions were developed for analyzing snails from two laboratory-maintained stocks. The analyses were performed using a total of 60 microsatellite primer sets and, for RAPD-PCR, a total of 19 random primers. We show that genetic diversity can readily be detected by both molecular methods among the field-collected snails from this small site. In addition, RAPD-PCR bands that were found in another study to segregate with parasite resistance were not detected in any of the field-collected snails analyzed.
ISSN: 00762997
Appears in Collections:Scopus 2001-2005

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