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Title: Development of piezoelectric DNA-based biosensor for direct detection of mycobacterium tuberculosis in clinical specimens
Authors: Thongchai Kaewphinit
Somchai Santiwatanakul
Chamras Promptmas
Kosum Chansiri
Keywords: Mycobacterium tuberculosis;Piezoelectric biosensor;Hybridization;Digestion genomic DNA;Open Access article
Issue Date: 2010
Citation: Sensors & Transducers Journal. Vol.113, No.2 (2010), 115-126
Abstract: This study was focused on establishment of piezoelectric biosensor for direct detection of Mycobacterium tuberculosis (MTB) in clinical specimens. The quartz crystal immobilized via 3-mercaptopropionic acid (MPA)/avidin/DNA biotinylated probe on gold surface and hybridization of the DNA target to DNA biotinylated probe. The optimal concentration of MPA, avidin and 5’-biotinylated DNA probe for immobilization of specific DNA probe on gold surface were 15 mM, 0.1 mg/ml and 1.5 μM, respectively. The detection of genomic DNA digestion in the range from 0.5 to 30 μg/ml. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex (MAC) and other microorganism. This target DNA preparation without amplification will reduce time consuming, costs, and the tedious step of amplification. This study can be extended to develop the new method which is high sensitivity, specificity, cheap, easy to use, and rapid for detection of MTB in many fields.
ISSN: 1726-5479
Appears in Collections:MT-Article

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