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dc.contributor.authorWalee Chamulitraten_US
dc.contributor.authorRainer Schmidten_US
dc.contributor.authorPascal Tomakidien_US
dc.contributor.authorWolfgang Stremmelen_US
dc.contributor.authorWarangkana Chungloken_US
dc.contributor.authorTsukasa Kawaharaen_US
dc.contributor.authorKazuhito Rokutanen_US
dc.contributor.otherGerman Cancer Research Centeren_US
dc.contributor.otherUniversitat Heidelbergen_US
dc.contributor.otherUniversitatsklinikum Heidelbergen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherTokushima Universityen_US
dc.identifier.citationOncogene. Vol.22, No.38 (2003), 6045-6053en_US
dc.description.abstractAmong five members of the NADPH oxidase (Nox) family, Nox1 confers mitogenic properties and is implicated to participate in the process of cell transformation. We have established two phenotypes of carcinogenesis model by ethanol treatment of human gingival keratinocytes immortalized with E6/E7 oncogenes of human papillomavirus type16: immortalized (EPI) nontransformed cells with epithelium-like morphology and more advanced transformed (FIB) cells with spindle fibroblastic-shape morphology. FIB membranes possessed a 63-kDa Nox1 protein at higher levels and exhibited 2.8-fold higher capability for superoxide and hydroxyl radical generation, compared with EPI membranes. Both EPI and FIB cells expressed more abundant Nox1 protein at a proliferating stage than that at a quiescent confluent phase. Immunofluorescence staining with an anti-Nox1 antibody showed that immunoreactive materials were distributed in the whole interior of both types of cells, while they were preferentially localized in the nuclei of FIB cells. Nuclei isolated from EPI and FIB cells contained a 63kDa-Nox1 protein. Compared with EPI cells, FIB cells expressed elevated levels of Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase proteins. Furthermore, JNK2 was constitutively phosphorylated in FIB cells. Together, our data strongly implicate Nox1 in redox-mediated signaling related to cellular activation of human keratinocytes at a more advanced stage of transformation.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleAssociation of gp91phox homolog Nox1 with anchorage-independent growth and MAP kinase-activation of transformed human keratinocytesen_US
Appears in Collections:Scopus 2001-2005

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