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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/2072
Title: Detection of non-amplified mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors
Authors: Thongchai Kaewphinit
Somchai Santiwatanakul
Chamras Promptmas
Kosum Chansiri
Keywords: Mycobacterium tuberculosis;piezoelectric biosensor;non-amplified genomic DNA;Open Access article
Issue Date: 2010
Citation: Sensors. Vol.10, 2010, 1846-1858
Abstract: Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a selfassembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/2072
ISSN: 1424-8220
Appears in Collections:MT-Article

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