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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/20742
Title: Stable integration and expression of mosquito-larvicidal genes from Bacillus thuringiensis subsp. israelensis and Bacillus sphaericus into the chromosome of Enterobacter amnigenus: A potential breakthrough in mosquito biocontrol
Authors: Sutipa Tanapongpipat
Nawarat Nantapong
Jeffrey Cole
Sakol Panyim
Thailand National Center for Genetic Engineering and Biotechnology
Mahidol University
University of Birmingham
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 25-Apr-2003
Citation: FEMS Microbiology Letters. Vol.221, No.2 (2003), 243-248
Abstract: Previously, we have successfully integrated a spectinomycin/streptomycin resistance gene into Enterobacter amnigenus strain An11, a potential host for mosquito control, using in vivo recombination via homologous recombination (An11S4::Ω). We now report the successful transfer of two mosquito-larvicidal genes, cry4B from Bacillus thuringiensis subsp. israelensis and binary toxin genes from Bacillus sphaericus, into the host genome. To facilitate the screening procedure, the E. amnigenus derivative, An11S4::Ω, was used as a host. The integration of both toxin genes by two successive crossover events interrupted the Ω region yielding two integrants designated An11S4::cry4B and An11S4::Ω::bin, respectively. Differences in the integration efficiency of these toxin genes were observed. The presence of both genes in the target sites of the host genome was verified by PCR. Cry4B was expressed weakly from An11S4::cry4B, but no expression of the binary toxin gene could be detected from An11S4::Ω::bin. Nevertheless, these two integrants exhibited mosquito-larvicidal activity against Aedes and Culex, suggesting that both proteins were expressed, but at very low levels. © 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0142030485&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/20742
ISSN: 03781097
Appears in Collections:Scopus 2001-2005

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