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Title: Physicochemical properties of amphotericin B liposomes prepared by reverse-phase evaporation method
Authors: Pleumchitt Rojanapanthu
Narong Sarisuta
Korakot Chaturon
Krisana Kraisintu
Mahidol University
Silpakorn University
Thailand Government Pharmaceutical Organization
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 24-Feb-2003
Citation: Drug Development and Industrial Pharmacy. Vol.29, No.1 (2003), 31-37
Abstract: The physicochemical properties of phosphatidylcholine-cholesterol liposomes containing amphotericin B and prepared by reverse-phase evaporation method were studied. Uniformly dispersed liposomal suspensions were obtained by employing 3:1 ratio (by volume) of diethyl ether to normal saline, 5 min sonication time at 7°C, and evaporation of diethyl ether at 25°C. Microscopic examination showed that the prepared liposomes were spheroids with unilamellar, oligolamellar, or multilamellar structure. The liposomes containing amphotericin B 2.0 mol% of total lipid led to the highest percentage of drug entrapment. Liposomes with maximum entrapment efficiency were obtained from using 250 μmol of total lipid. The liposomal amphotericin B possessing the highest drug entrapment efficiency (approximately 95%) with particle size range of 1307-1451 nm was the one composed of 1:1 molar ratio of phosphatidylcholine to cholesterol.
ISSN: 03639045
Appears in Collections:Scopus 2001-2005

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