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Title: CpG DNA, liposome and refined antigen oral cholera vaccine
Authors: Ratree Leelawongtawon
Srinuan Somroop
Urai Chaisri
Pongsri Tongtawe
Manas Chongsa-nguan
Thareerat Kalambaheti
Pramuan Tapchaisri
Sathit Pichyangkul
Yuwaporn Sakolvaree
Hisao Kurazono
Hideo Hayashi
Wanpen Chaicumpa
Mahidol University
Thammasat University
Armed Forces Research Institute of Medical Sciences, Thailand
Okayama University
University of Tsukuba
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Dec-2003
Citation: Asian Pacific Journal of Allergy and Immunology. Vol.21, No.4 (2003), 231-239
Abstract: An oral cholera vaccine made up of three Vibrio cholerae antigens, i.e. lipopolysaccharide (LPS), recombinant toxin co-regulated pili (rTcpA) and heat-treated cholera toxin (H-CT) has been developed in six different formulations. Eight-week-old Wistar rats were divided into nine groups and immunized as follows: the first group received the oral vaccine 1 consisting of the three antigens (LPS, rTcpA and H-CT) associated with a liposome (L) and bacterial CpG-DNA (ODN#1826). The rats of groups 2 and 3 received oral vaccines 2 and 3 consisting of the liposome-associated three antigens with and without non-bacterial CpG-DNA (ODN#1982), respectively. Rats of groups 4 received oral vaccine 4 consisting of the three antigens mixed with the ODN#1826, similar to vaccine 1, but without liposome. Rats of groups 5 and 6 received oral vaccines 5 and 6 consisting of the three antigens with and without ODN#1982, respectively, similar to vaccines 2 and 3, but without liposome. Rats of groups 7, 8 and 9 received oral placebos, namely liposomes (L), ODN#1826 (CpG), and vaccine diluent, i.e. 5% NaHCO3solution, respectively. All vaccines were given in three doses at 14-day intervals. It was found that the combination of liposome and ODN#1826 in vaccine 1 evoked the highest immune response to V. cholerae antigen compared to other vaccine formulations and placebos, as measured by the appearance of antigen-specific antibody-producing cells in the intestinal lamina propria. The immunogenicity according to the magnitude of the immune response was: V1>V2=V3>V4>V5=V6>V7=V8=V9. The results of this study indicate that CpG-DNA and liposome are effective mucosal adjuvants for an oral cholera vaccine prepared from refined V. cholerae antigens and their combination seems to be synergistic. The potential role of liposome as a vaccine delivery vehicle has been confirmed.
ISSN: 0125877X
Appears in Collections:Scopus 2001-2005

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