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dc.contributor.authorP. Utaisincharoenen_US
dc.contributor.authorW. Kespichayawattanaen_US
dc.contributor.authorN. Anuntagoolen_US
dc.contributor.authorP. Chaisuriyaen_US
dc.contributor.authorS. Pichyangkulen_US
dc.contributor.authorA. M. Kriegen_US
dc.contributor.authorS. Sirisinhaen_US
dc.contributor.otherChulabhorn Research Instituteen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherArmed Forces Research Institute of Medical Sciences, Thailanden_US
dc.contributor.otherPfizer Inc.en_US
dc.identifier.citationClinical and Experimental Immunology. Vol.132, No.1 (2003), 70-75en_US
dc.description.abstractUnmethylated CpG motif in synthetic oligodeoxynucleotide (CpG ODN) or bacterial DNA is well recognized for its role in innate immunity, including enhancing production of NO and cytokines by macrophages. In the present study, we demonstrated the effect of CpG ODN on the phagocytic uptake of bacteria by macrophages. Flow cytometric analysis of mouse macrophages (RAW 264.7) incubated with fluorescein isothiocyanate (FITC)-labelled Burkholderia pseudomallei, Salmonella enterica serovar Typhi or Escherichia coli showed that CpG ODN increased the uptake of these bacteria by mouse macrophages. The enhancement of bacterial uptake by CpG ODN was concentration-dependent. The increase of bacterial uptake by CpG ODN-activated macrophages shown above is consistent with the result of bacteria internalization study using a standard antibiotic protection assay. There was also an increase in the rate and degree of multi-nucleated giant cell formation, phenomena which have been shown previously to be unique when the cells were infected with B. pseudomallei. These observations may provide significant insights for future investigation into host cell-pathogen interaction.en_US
dc.rightsMahidol Universityen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleCpG ODN enhances uptake of bacteria by mouse macrophagesen_US
Appears in Collections:Scopus 2001-2005

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