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Title: Cucurbitacin B causes increased radiation sensitivity of human breast cancer cells via G2/M cell cycle arrest
Authors: Suwit Duangmano
Phorntip Sae-lim
Apichart Suksamrarn
Pimpichaya Patmasiriwat
Frederick E. Domann
Keywords: Cucurbitacin B;Human Breast Cancer;Cell Cycle Arrest;Radiation Sensitivity
Issue Date: 17-Jun-2013
Publisher: Mahidol University. Faculty of Medical Technology
Citation: Journal of Oncology. 2012, ID 601682
Abstract: Purpose. To explore the effects of cucurbitacin B on the radiation survival of human breast cancer cells and to elucidate the cellular mechanism of radiosensitization if any. Materials and Methods. Human breast carcinoma cell lines were treated with cucurbitacin B before irradiation with 0–10Gy of 137Cs gamma rays. The effect of cucurbitacin B on cell-survival following irradiation was evaluated by colony-forming assay. Cell cycle distributions were investigated using flow cytometry. Real-time PCR and western blots were performed to investigate the expression of cell cycle checkpoints. Results. Cucurbitacin B inhibited breast cancer cell proliferation in a dose-dependent manner. Only MDA-MB-231 and MCF7:5C cells but not SKBR-3 cells were radiosensitized by cucurbitacin B. Flow cytometric analysis for DNA content indicated that cucurbitacin B resulted in G2/M arrest in MDA-MB-231 andMCF7:5C but not SKBR-3 cells.Moreover, Real-time PCR and western blot analysis demonstrated upregulated p21 expression before irradiation, a likely cause of the cell cycle arrest. Conclusion. Taken together, these findings suggest that cucurbitacin B causes radiosensitization of some breast cancer cells, and that cucurbitacin B induced G2/M arrest is an importantmechanism. Therefore, combinations of cucurbitacin B with radiotherapy may be appropriate for experimental breast cancer treatment.
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