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Title: Growth factor combination for chondrogenic induction from human mesenchymal stem cell
Authors: Nitaya Indrawattana
Guoping Chen
Mika Tadokoro
Linzi H. Shann
Hajime Ohgushi
Tetsuya Tateishi
Junzo Tanaka
Ahnond Bunyaratvej
National Institute of Advanced Industrial Science and Technology
Mahidol University
National Institute for Materials Science Tsukuba
The Institute of Science and Technology for Research and Development, Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 30-Jul-2004
Citation: Biochemical and Biophysical Research Communications. Vol.320, No.3 (2004), 914-919
Abstract: During the last decade, many strategies for cartilage engineering have been emerging. Stem cell induction is one of the possible approaches for cartilage engineering. The mesenchymal stem cells (MSCs) with their pluripotency and availability have been demonstrated to be an attractive cell source. It needs the stimulation with cell growth factors to make the multipluripotent MSCs differentiate into chondrogenic lineage. We have shown particular patterns of in vitro chondrogenesis induction on human bone marrow MSCs (hBMSCs) by cycling the growth factors. The pellet cultures of hBMSCs were prepared for chondrogenic induction. Growth factors: TGF-β3, BMP-6, and IGF-1 were used in combination for cell induction. Gene expression, histology, immunohistology, and real-time PCR methods were measured on days 21 after cell induction. As shown by histology and immunohistology, the induced cells have shown the feature of chondrocytes in their morphology and extracellular matrix in both inducing patterns of combination and cycling induction. Moreover, the real-time PCR assay has shown the expression of gene markers of chondrogenesis, collagen type II and aggrecan. This study has demonstrated that cartilage tissue can be created from bone marrow mesenchymal stem cells. Interestingly, the combined growth factors TGF-β3 and BMP-6 or TGF-β3 and IGF-1 were more effective for chondrogenesis induction as shown by the real-time PCR assay. The combination of these growth factors may be the important key for in vitro chondrogenesis induction. © 2004 Elsevier Inc. All rights reserved.
ISSN: 0006291X
Appears in Collections:Scopus 2001-2005

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