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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/21182
Title: Bacillus thuringiensis Cry4A and Cry4B mosquito-larvicidal proteins: Homology-based 3D model and implications for toxin activity
Authors: Chanan Angsuthanasombat
Panapat Uawithya
Somphob Leetachewa
Walairat Pornwiroon
Puey Ounjai
Teerakiat Kerdcharoen
Gerd Katzenmeier
Sakol Panyim
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 31-May-2004
Citation: Journal of Biochemistry and Molecular Biology. Vol.37, No.3 (2004), 304-313
Abstract: Three-dimensional (3D) models for the 65-kDa activated Cry4A and Cry4B δ-endotoxins from Bacillus thuringienis subsp. israelensis that are specifically toxic to mosquito-larvae were constructed by homology modeling, based on atomic coordinates of the CrylAa and Cry3Aa crystal structures. They were structurally similar to the known structures, both derived 3D models displayed a three-domain organization: the N-terminal domain (I) is a seven-helix bundle, while the middle and C-terminal domains are primarily comprise of anti-parallel β-sheets. Circular dichroism spectroscopy confirmed the secondary structural contents of the two homology-based Cry4 structures. A structural analysis of both Cry4 models revealed the following: (a) Residues Arg-235 and Arg-203 are located in the interhelical 5/6 loop within the domain I of Cry4A and Cry4B, respectively. Both are solvent exposed. This suggests that they are susceptible to tryptic cleavage. (b) The unique disulphide bond, together with a proline-rich region within the long loop connecting α4 and α5 of Cry4A, were identified. This implies their functional significance for membrane insertion. (c) Significant structural differences between both models were found within domain II that may reflect their different activity spectra. Structural insights from this molecular modeling study would therefore increase our understanding of the mechanic aspects of these two closely related mosquito-larvicidal proteins.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=2642587327&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/21182
ISSN: 12258687
Appears in Collections:Scopus 2001-2005

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