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Title: High-throughput cloning of Campylobacter jejuni ORFs by in vivo recombination in Escherichia coli
Authors: Jodi R. Parrish
Thawornchai Limjindaporn
Julie A. Hines
Jiayou Liu
Guozhen Liu
Russell L. Finley
Wayne State University School of Medicine
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry
Issue Date: 1-May-2004
Citation: Journal of Proteome Research. Vol.3, No.3 (2004), 582-586
Abstract: A rate-limiting and costly step in many proteomics analyses is the cloning of all of the ORFs for an organism into technique-specific vectors. Here, we describe the generation of a Campylobacter jejuni expression clone set using a high-throughput cloning approach based on recombination in E. coli. The approach uses native E. coli recombination functions and requires no in vitro enzymatic steps or special strains. Our results indicate that this approach is an efficient and economical alternative for high-throughput cloning.
ISSN: 15353893
Appears in Collections:Scopus 2001-2005

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