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Title: Epidemiological and Molecular Characterization of Dengue Virus Circulating in Bhutan, 2013-2014
Authors: Sangay Zangmo
Chonticha Klungthong
Piyawan Chinnawirotpisan
Srisurang Tantimavanich
Nathamon Kosoltanapiwat
Butsaya Thaisomboonsuk
Kelzang Phuntsho
Sonam Wangchuk
In-Kyu Yoon
Stefan Fernandez
Mahidol University. Faculty of Medical Technology. Department of Clinical Microbiology and Applied Technology
Keywords: Open Access article
Issue Date: 21-Aug-2015
Citation: Plos Neglected Tropical Diseases. Vol.9, No.8 (2015), 1-14
Abstract: Dengue is one of the most significant public health problems in tropical and subtropical countries, and is increasingly being detected in traditionally non-endemic areas. In Bhutan, dengue virus (DENV) has only recently been detected and limited information is available. In this study, we analyzed the epidemiological and molecular characteristics of DENV in two southern districts in Bhutan from 2013–2014. During this period, 379 patients were clinically diagnosed with suspected dengue, of whom 119 (31.4%) were positive for DENV infection by NS1 ELISA and/or nested RT-PCR. DENV serotypes 1, 2 and 3 were detected with DENV-1 being predominant. Phylogenetic analysis of DENV-1 using envelope gene demonstrated genotype V, closely related to strains from northern India.
Appears in Collections:MT-Article

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