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Title: Performance of four sources of cholesterol oxidase for serum cholesterol determination by the enzymatic endpoint method
Authors: Porntip H. Lolekha
Pornpen Srisawasdi
Patcharee Jearanaikoon
Nuanchawee Wetprasit
Busarawan Sriwanthana
Martin H. Kroll
Mahidol University
Khon Kaen University
Ramkhamheng University
National Institutes of Health, Bethesda
UT Southwestern Medical School
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 1-Jan-2004
Citation: Clinica Chimica Acta. Vol.339, No.1-2 (2004), 135-145
Abstract: Background: Cholesterol oxidase is used for the determination of serum cholesterol. It can be derived from Streptomyces, Pseudomonas fluorescens, Cellulomonas, and Brevibacterium. This study compared the performance characteristics of four enzymes in the endpoint cholesterol determination. Methods: Using the Mega analyzer, we studied assay optimization, linearity, precision, recovery, interference, stability, and compared 110 patient samples. Results: The linearity for the four enzymes was up to 13.0 mmol/l at the optimal enzyme activity. The average within-run CVs ranged from 1.6% to 1.9% and between-day ranged from 2.8% to 3.0%, within the NCEP analytical criteria. The analytical recoveries obtained from four reagents (∼ 96.5%) were excellent. The assays using these enzyme sources compared favorably with the commercial method and appeared accurate near the clinical decision cut-points. Hemoglobin concentration at 1.9 g/l interfered with the P. fluorescens cholesterol oxidase. Bilirubin caused a negative interference while lipemia generated a positive interference with all enzyme sources. Reagents were stable up to 6 weeks. Conclusions: Streptomyces, Cellulomonas, and Brevibacterium were essentially analytically equivalent. Streptomyces and Cellulomonas cholesterol oxidase are one-quarter as expensive Brevibacterium. Cellulomonas is a new source of cholesterol oxidase for determining serum cholesterol by the endpoint method. © 2003 Elsevier B.V. All rights reserved.
ISSN: 00098981
Appears in Collections:Scopus 2001-2005

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