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Title: Monocyte activation by Porphyromonas gingivalis LPS in aggressive periodontitis with the use of whole-blood cultures
Authors: R. Mahanonda
N. Sa-Ard-Iam
O. Charatkulangkun
A. Promsudthi
R. E. Schifferle
K. Yongvanichit
S. Pichyangkul
Chulalongkorn University
Mahidol University
University at Buffalo, State University of New York
Armed Forces Research Institute of Medical Sciences, Thailand
Keywords: Dentistry
Issue Date: 1-Jan-2004
Citation: Journal of Dental Research. Vol.83, No.7 (2004), 540-545
Abstract: In this study, we re-visited the issue of hyper-responsiveness of monocytes to bacterial lipopolysaccharide (LPS) in aggressive periodontitis patients. We used whole-blood cultures to compare monocyte activation by Porphyromonas gingivalis LPS between Thai subjects with generalized aggressive periodontitis and those without periodontitis. Upon stimulation with P. gingivalis LPS, expression of co-stimulatory molecules on monocytes and expression of CD69 on NK and γδ T-cells were analyzed by flow cytometry, and the production of interleukin-1β and prostaglandin E2was monitored by ELISA. LPS stimulation resulted in a dose-dependent up-regulation of CD40, CD80, and CD86 on monocytes, and up-regulation of CD69 on NK cells and γδ T-cells in both the periodontitis and non-periodontitis groups. The levels of activation markers and the mediator production after LPS stimulation were quite similar for both groups. In conclusion, we did not observe hyper-responsiveness of monocytes to P. gingivalis LPS challenge in Thai patients with aggressive periodontitis.
ISSN: 00220345
Appears in Collections:Scopus 2001-2005

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