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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/21362
Title: The gene encoding the nucleocapsid protein of Gill-associated nidovirus of Penaeus monodon prawns is located upstream of the glycoprotein gene
Authors: Jeff A. Cowley
Lee C. Cadogan
Kirsten M. Spann
Nusra Sittidilokratna
Peter J. Walker
CSIRO Livestock Industries
Mahidol University
National Institute of Allergy and Infectious Diseases
Keywords: Immunology and Microbiology
Issue Date: 1-Aug-2004
Citation: Journal of Virology. Vol.78, No.16 (2004), 8935-8941
Abstract: The ORF2 gene of Gill-associated virus (GAV) of Penaeus monodon prawns resides 93 nucleotides downstream of the ORF1a-ORF1b gene and encodes a 144-amino-acid hydrophilic polypeptide (15,998 Da; pI, 9.75) containing 20 basic (14%) and 13 acidic (9%) residues and 19 prolines (13%). Antiserum to a synthetic ORF2 peptide or an Escherichia coli-expressed glutathione S-transferase-ORF2 fusion protein detected a 20-kDa protein in infected lymphoid organ and gill tissues in Western blots. The GAV ORF2 fusion protein antiserum also cross-reacted with the p20 nucleoprotein in virions of the closely related Yellow head virus. By immuno-gold electron microscopy, it was observed that the ORF2 peptide antibody localized to tubular GAV nucleocapsids, often at the ends or at lateral cross sections. As GAV appears to contain only two structural protein genes (ORF2 and ORF3), these data indicate that GAV differs from vertebrate nidoviruses in that the gene encoding the nucleocapsid protein is located upstream of the gene encoding the virion glycoproteins.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=3543096771&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/21362
ISSN: 0022538X
Appears in Collections:Scopus 2001-2005

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