Please use this identifier to cite or link to this item:
Title: SERF: A new antigen in the Cromer blood group system
Authors: J. Banks
J. Poole
N. Ahrens
A. Seltsam
A. Salama
K. Hue-Roye
J. R. Storry
P. Palacajornsuk
B. W. Ma
D. M. Lublin
Marion E. Reid
National Blood Service
Charité – Universitätsmedizin Berlin
New York Blood Center
Mahidol University
Washington University School of Medicine in St. Louis
Hanover Medical College
Lunds Universitet
Keywords: Immunology and Microbiology
Issue Date: 1-Aug-2004
Citation: Transfusion Medicine. Vol.14, No.4 (2004), 313-318
Abstract: The Cromer blood group system consists of eight high incidence and three low incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high incidence antigen, named SERF. Sequence analyses of DNA from a Thai female whose serum contained the alloantibody to a high incidence antigen in the Cromer blood group system (anti-SERF) and from her two children were performed. Reverse transcriptase-polymerase chain reaction (RT-PCR) and sequence analysis on cDNA from the proband and PCR-restriction fragment length polymorphism analysis on DNA from Thais were also performed. To map the epitope, DAF deletion mutants were tested by immunoblotting with anti-SERF. Sequence analysis revealed a substitution of 647C>T in exon 5 DAF in the proband. The proband's two children and two of 100 Thais were heterozygotes 647C/T. Analysis using DAF deletion mutants revealed the antigenic determinant to be within short consensus repeat 3 (SCR3), which is encoded by exon 5. This study describes a novel high incidence antigen (SERF) in the Cromer blood group system characterized by the amino acid proline at position 182 in SCR3 of DAF. The SERF-negative proband has a substitution mutation that predicts for leucine at this position. SERF has been provisionally assigned the International Society of Blood Transfusion number 021-012(CROM 12).
ISSN: 09587578
Appears in Collections:Scopus 2001-2005

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.