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Title: Cloning and characterization of a mosquito larvicidal toxin produced during vegetative stage of Bacillus sphaericus 2297
Authors: Boonhiang Promdonkoy
Patcharee Promdonkoy
Sutipa Tanapongpipat
Plearnpis Luxananil
Namchai Chewawiwat
Mongkon Audtho
Sakol Panyim
Thailand National Center for Genetic Engineering and Biotechnology
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Jan-2004
Citation: Current Microbiology. Vol.49, No.2 (2004), 84-88
Abstract: The mosquitocidal toxin 1 (mtx1) gene from genomic DNA of B. sphaericus strain 2297 was cloned and expressed in E. coli. DNA sequencing analysis of the cloned gene revealed a single open reading frame encoding an 870-amino acid polypeptide. Expression level of the full-length gene in E. coli was very low even though strong promoter was used or the gene was expressed as a fusion protein. Expression level was highly improved after the putative leader sequence was deleted, and the truncated gene was expressed as a fusion protein with glutathione S-transferase (GST-tMtx1). E. coli cells expressing GST-tMtx1 was highly toxic to Culex quinquefasciatus larvae and showed lower toxicity against Anopheles dirus and Aedes aegypti larvae. Enterobacter amnigenus An11, a mosquito larval gut colonizable bacteria, transformed with the cloned gene exhibited mosquito larvicidal activity. Result suggested that there is a potential to develop this protein to be used as an alternative mosquito control agent.
ISSN: 03438651
Appears in Collections:Scopus 2001-2005

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