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Title: The use of flow cytometry as a diagnostic test for malaria parasites
Authors: Varee Wongchotigul
Nirut Suwanna
Srivicha Krudsood
Duangrudee Chindanond
Shigeyuki Kano
Nobuaki Hanaoka
Yasumasa Akai
Yasunori Maekawa
Satoshi Nakayama
Somei Kojima
Sornchai Looareesuwan
Mahidol University
National Center for Global Health and Medicine
Sysmex Corporation
Keywords: Medicine
Issue Date: 1-Sep-2004
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.35, No.3 (2004), 552-559
Abstract: A total of 453 clinical blood samples were determined for malaria parasites by flow cytometric assay (FCM) and reagents from Sysmex Corporation, Japan. In this study, the FCM greatly simplified and accelerated parasite detection, with sensitivity of 91.26%, specificity 86.28% and accuracy 87.42%. Overall, the parasite counts by flow cytometric measurement correlated well with the parasitemia measured by microscopic assay (regression coefficient = 0.9409). The detection limit was 0.05-0.1% parasitemia. No evidence of malaria parasites in either blood donor volunteers or other disease patients groups was determined by FCM. However, 48 samples who had been treated with antimalarial drugs and whose parasite microscopic counts were negative, showed false-positive results. When the data of these 48 samples were analyzed, they were found to have high levels of reticulocytes, ranging from 2.0-18.9%. This finding suggested that a high reticulocyte concentration in the blood may interfere with the performance of the FCM. Further improvement, by eliminating this interference, will make the FCM one of the most promising tests for malaria diagnosis.
ISSN: 01251562
Appears in Collections:Scopus 2001-2005

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